Preparation of sludge-based micro-electrolysis filler and its application in pharmaceutical wastewater treatment by an up-flow aerated filter.

Sewage sludge (SS) and raw pharmaceutical wastewater (RPW) are both toxic and harmful wastes, which are a menace to human and animal health and the ecosystem. A sludge-base micro-electrolysis filler (SMEF) was gained by SS and Fe powder as the primary raw materials. The preparation process of the SMEF was achieved based on the tetracycline hydrochloride (TCH) removal efficiency. The physicochemical characteristics (e.g., surface area, morphology features, function groups, and valence state of Fe) of the obtained SMEF were decided. With an Fe/SS ratio of 1/2, a sintering temperature of 1050 °C, a sintering time of 30 min, an initial pH of 3, and a filler dosage of 100 g/L, the SMEF demonstrated a high degradation ability for TCH with a removal rate reached 95.62% in 24 h. Kinetic analysis showed that the adsorption process of TCH was consistent with the pseudo-first-order Lagergren kinetic model. Moreover, degradation mechanism analysis showed that TCH was gradually degraded through dehydroxylation, demethylation, ring opening, oxidation, and reduction in solution. The SMEF had a good continuous removal performance for contaminants in RPW in an up-flow aerated filter. The removal efficiency of TOC and TN reached 46.60 and 42.27% within 24 h, respectively. The treated pharmaceutical wastewater was considered non-biotoxic after 24-h treatment with the SMEF. This study presents a innovative Fe-C micro-electrolysis filler based on SS and is important to the environmental-friendly recycling of SS and sustainable treatment of RPW, achieving the purpose of waste disposal.

Encapsulins from Ca. Brocadia fulgida: An effective tool to enhance the tolerance of engineered bacteria (pET-28a-cEnc) to Zn2.

Zn2+ is largely discharged from many industries and poses a severe threat to the environment, making its remediation crucial. Encapsulins, proteinaceous nano-compartments, may protect cells against environmental stresses by sequestering toxic substances. To determine whether hemerythrin-containing encapsulins (cEnc) from anammox bacteria Ca. Brocadia fulgida can help cells deal with toxic substances such as Zn2+, we transferred cEnc into E.coli by molecular biology technologies for massive expression and then cultured them in media with increasing Zn2+ levels. The engineered bacteria (with cEnc) grew better and entered the apoptosis phase later, while wild bacteria showed poor survival. Furthermore, tandem mass tag-based quantitative proteomic analysis was used to reveal the underlying regulatory mechanism by which the genetically-engineered bacteria (with cEnc) adapted to Zn2+ stress. When Zn2+ was sequestered in cEnc as a transition, the engineered bacteria presented a complex network of regulatory systems against Zn2+-induced cytotoxicity, including functions related to ribosomes, sulfur metabolism, flagellar assembly, DNA repair, protein synthesis, and Zn2+ efflux. Our findings offer an effective and promising stress control strategy to enhance the Zn2+ tolerance of bacteria for Zn2+ remediation and provide a new application for encapsulins.

Anammox and partial denitrification coupling: a review.

As a new wastewater biological nitrogen removal process, anammox and partial denitrification coupling not only plays a significant role in the nitrogen cycle, but also holds high engineering application value. Because anammox and some denitrifying bacteria are coupled under harsh living conditions, certain operating conditions and mechanisms of the coupling process are not clear; thus, it is more difficult to control the process, which is why the process has not been widely applied. This paper analyzes the research focusing on the coupling process in recent years, including anammox and partial denitrification coupling process inhibitors such as nitrogen (NH4 +, NO2 -), organics (toxic and non-toxic organics), and salts. The mechanism of substrate removal in anammox and partial denitrification coupling nitrogen removal is described in detail. Due to the differences in process methods, experimental conditions, and sludge choices between the rapid start-up and stable operation stages of the reactor, there are significant differences in substrate inhibition. Multiple process parameters (such as pH, temperature, dissolved oxygen, redox potential, carbon-to-nitrogen ratio, and sludge) can be adjusted to improve the coupling of anammox and partial denitrification to modify nitrogen removal performance.